Histological samples should be fixed in Bouin's solution, Helly's solution or 10% buffered formalin. Fix live fish after anesthetized. Use 10 moribund fish and 10 that are apparently normal from the same lot. Dead fish are unsuitable for histology. The volume of fixative must be 10 times the volume of tissue. For fish longer than 6 cm, slit the abdomen, detach the intestine at the anus, and pull the internal organ mass out slightly. For large fish, send only specified organs in fixative. Call the FPS for specific instructions prior to fixing. Further details regarding necropsy and histologic sampling for both finfish and shellfish are provided in the histology chapter (Chapter 6) of this manual.
Sample sizes for shellfish are: 30 live adult animals for a disease history; 60 live adults, 200 live spat and 1,000 live larvae for certification for import of seed from an out-of-state Pacific oyster stock; maximum of 10 live or fixed animals for diagnostic purposes.
Sample sizes for shellfish are: 30 live adult animals for a disease history; 60 live adults, 200 live spat and 1,000 live larvae for certification for import of seed from an out-of-state Pacific oyster stock; maximum of 10 live or fixed animals for diagnostic purposes.
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