Sunday, February 27, 2011

Finfish Bacteriology

Small fish must be received either alive or freshly dead (within 1-2 hours) on blue ice in a cooler. Fish must not be frozen. Bagged fish should not be in direct contact with blue ice or they will freeze.
Live fish are preferred for diagnostic samples. At least 10 moribund fish should be placed in one or more large leak-proof plastic bags containing hatchery water. Seal the bags so space for air remains and leakage will not occur. Label bags with fish status (moribund or healthy), incubator or raceway number, stock and species and enclose a Sample Submission Form (see page 1-8) with each shipment. If oxygen is available, add to bags before sealing. Addition of an oxygen tablet to each bag is recommended, particularly for samples that must be shipped. Make a similar bag containing 10 healthy fish. Again, if the fish are large fingerlings or smolts, the amount of fish per bag should be adjusted accordingly.
In addition, enclose 10 moribund fish with a damp paper towel in a smaller dry plastic bag. Do not add water. If the live fish do not survive transport, then the "dry" fish, which will have undergone less deterioration and contamination from the water and its bacterial flora, will be processed instead. In a disease outbreak, 30 fish per lot of affected fish will be required for shipment (10 moribund, 10 healthy and 10 moribund, but dry).

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